Arthroscopy: The Journal of Arthroscopic and Related Surgery
Volume 25, Issue 12 , Pages 1435-1441 , December 2009

Augmentation of Degenerated Human Cartilage In Vitro Using Magnetically Labeled Mesenchymal Stem Cells and an External Magnetic Device

  • Takaaki Kobayashi, M.D.

      Affiliations

    • Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • ,
  • Mitsuo Ochi, M.D., Ph.D.

      Affiliations

    • Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • Corresponding Author InformationAddress correspondence and reprint requests to Mitsuo Ochi, M.D., Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
    • ,
  • Shinobu Yanada, M.Sc.

      Affiliations

    • Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • ,
  • Masakazu Ishikawa, M.D., Ph.D.

      Affiliations

    • Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • ,
  • Nobuo Adachi, M.D., Ph.D.

      Affiliations

    • Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • ,
  • Masataka Deie, M.D., Ph.D.

      Affiliations

    • Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • ,
  • Koji Arihiro, M.D., Ph.D.

      Affiliations

    • Department of Anatomical Pathology, Hiroshima University Hospital, Hiroshima, Japan

Received 5 October 2008 ,Accepted 10 June 2009.

References 

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  2. Murphy JM, Fink DJ, Hunziker EB, Barry FP. Stem cell therapy in a caprine model of osteoarthritis. Arthritis Rheum. 2003;48:3464–3474
  3. Nishimori M, Deie M, Kanaya A, Exham H, Adachi N, Ochi M. Repair of chronic osteochondral defects in the rat (A bone marrow-stimulating procedure enhanced by cultured allogenic bone marrow mesenchymal stromal cells). J Bone Joint Surg Br. 2006;88:1236–1244
  4. Wakitani S, Imoto K, Yamamoto T, Saito M, Murata N, Yoneda M. Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. Osteoarthritis Cartilage. 2002;10:199–206
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  6. Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418:41–49
  7. Agung M, Ochi M, Yanada S, et al. Mobilization of bone marrow-derived mesenchymal stem cells into the injured tissues after intraarticular injection and their contribution to tissue regeneration. Knee Surg Sports Traumatol Arthrosc. 2006;14:1307–1314
  8. Ochi M, Adachi N, Nobuto H, Yanada S, Ito Y, Agung M. Articular cartilage repair using tissue engineering technique—Novel approach with minimally invasive procedure. Artif Organs. 2004;28:28–32
  9. Kobayashi T, Ochi M, Yanada S, et al. A novel cell delivery system using magnetically labeled mesenchymal stem cells and an external magnetic device for clinical cartilage repair. Arthroscopy. 2008;24:69–76
  10. Outerbridge RE. The etiology of chondromalacia patellae. J Bone Joint Surg Br. 1961;43:752–757
  11. Mankin HJ. Biochemical and metabolic aspects of osteoarthritis. Orthop Clin North Am. 1971;2:19–31
  12. Yanada S, Ochi M, Adachi N, et al. Effects of CD44 antibody– or RGDS peptide–immobilized magnetic beads on cell proliferation and chondrogenesis of mesenchymal stem cells. J Biomed Mater Res A. 2006;77:773–784
  13. Motoyama M, Deie M, Ochi M, et al. In vitro cartilage formation using TGF–immobilized magnetic beads and mesenchymal stem cell-magnetic bead complexes under magnetic field conditions. J Biomed Mater Res A. 2009 Jan 26;[Epub ahead of print.]
  14. Hamasaki T, Tanaka N, Ochi M, et al. Characterization of labeled neural progenitor cells for magnetic targeting. Neuroreport. 2005;16:1641–1645
  15. Nishida K, Tanaka N, Ochi M, et al. Magnetic targeting of bone marrow stromal cells into spinal cord: Through cerebrospinal fluid. Neuroreport. 2006;17:1269–1272
  16. Hamasaki T, Tanaka N, Ochi M, et al. Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic cocultures. Spine. 2007;32:2300–2305
  17. Sugioka T, Ochi M, Yasunaga Y, Adachi N, Yanada S. Accumulation of magnetically labeled rat mesenchymal stem cells using an external magnetic force, and their potential for bone regeneration. J Biomed Mater Res A. 2008;85:597–604

 Supported in part by grants-in-aid to M.O. from the Japan Ministry of Education, Culture, Sports, Science and Technology (No. 16209045).

 The authors report no conflict of interest.

PII: S0749-8063(09)00521-0

doi: 10.1016/j.arthro.2009.06.009

Arthroscopy: The Journal of Arthroscopic and Related Surgery
Volume 25, Issue 12 , Pages 1435-1441 , December 2009

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