Purpose
To evaluate the effects of vigorous short-term exercise on the platelet and other
cellular components of 2 point-of-care blood-processing devices: a buffy coat–based
platelet-rich plasma (PRP) product and a plasma-based PRP product.
Methods
Twenty healthy subjects (aged 21-45 years) participated in a 20-minute vigorous exercise
regimen on an upright stationary bike at 70% to 85% of maximum target heart rate.
Pre- and post-exercise blood was processed in either a plasma-based or automated buffy
coat–based PRP system. Complete blood counts were used to compare the cellular components
in whole blood and the PRP products.
Results
Exercise significantly increased the concentrations of platelets by over 20% in whole
blood (P < .001) and in both PRP products (P = .002 and P = .018). Both devices performed consistently with pre- and post-exercise blood. Buffy
coat–based PRP prepared after exercise was also significantly larger in volume and
had a significantly higher concentration of mobilized hematopoietic stem cells (hematopoietic
progenitor cells [HPCs], from 1.7/μL to 2.7/μL, P = .043). The concentrations of all white blood cell types were increased, which could
be differentially collected in the devices studied.
Conclusions
Exercise can be used to consistently alter the composition of PRP. Twenty minutes
of vigorous exercise can increase platelet concentrations in plasma-based and buffy
coat–based PRP products and can increase HPC concentrations and volume in buffy coat–based
PRP.
Clinical Relevance
This study shows a nonpharmacologic method to increase platelet and HPC harvests from
peripheral blood. This is important because it highlights a method for altering biological
therapies with limited comorbidity.
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Article info
Publication history
Accepted:
June 11,
2018
Received:
December 7,
2017
Footnotes
The authors report the following potential conflict of interest or source of funding: This study was funded by and performed at Arthrex. A.W.A. reports speaking and consulting fees from Arthrex. M.F.R-C., M.S.T., and A.N. were employees of Arthrex Inc at the time the study was performed. R.M.H. and A.M.M. are currently employees of Arthrex Inc. Full ICMJE author disclosure forms are available for this article online, as supplementary material.
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