Purpose
To investigate the influence of patient demographics and rotator cuff tear characteristics
on the cellular proliferation potential of subacromial bursa–derived cells (SBDCs).
Methods
Patients undergoing arthroscopic rotator cuff repair between December 2017 and February
2019 were considered for enrollment in the study. Basic demographic information as
well as medical and surgical history were obtained for each patient. Subacromial bursa
was harvested from over the rotator cuff tendon. Cellular proliferation was evaluated
after 3 weeks of incubation by counting nucleated SBDCs. Fluorescence-activated cell
sorting (FACS) analysis was performed to confirm the presence of mesenchymal stem
cell (MSC) specific surface markers. Using preoperative radiographs and magnetic resonance
imaging (MRI), acromiohumeral distance (AHD), severity of cuff tear arthropathy, and
rotator cuff tear characteristics were evaluated.
Results
Seventy-three patients (mean age: 57.2 ± 8.5 years) were included in the study. There
was no significant difference in cellular proliferation of SBDCs when evaluating the
influence of age, sex, body mass index (BMI), smoking status, and presence of systemic
comorbidities (p > .05, respectively). Similarly, there was no significant difference in cellular
proliferation of SBDCs when looking at rotator cuff tear characteristics (size, tendon
retraction, fatty infiltration, muscle atrophy), AHD, or severity of cuff tear arthropathy
(p > .05). FACS analysis confirmed nucleated SBDCs to have a high positive rate of MSC
specific surface markers.
Conclusion
Subacromial bursa consistently demonstrated a high cellular proliferation potential
regardless of patient demographics, rotator cuff tear characteristics, and severity
of glenohumeral joint degeneration.
Clinical Relevance
These findings may alleviate concerns that subacromial bursa might lose cellular proliferation
potential when being used for biologic augmentation in massive and degenerated rotator
cuff tears, thus assisting in predicting tendon healing and facilitating surgical
decision-making.
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Article info
Publication history
Published online: June 14, 2020
Accepted:
June 4,
2020
Received:
December 8,
2019
See commentary on page 2803Footnotes
The authors report the following potential conflicts of interest or sources of funding: M.P.C. reports personal fees from the Arthroscopy Association of North America. A.D.M. receives research support from and is a consultant for Arthrex. Full ICMJE author disclosure forms are available for this article online, as supplementary material.
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© 2020 by the Arthroscopy Association of North America
