Use of a Hyperosmolar Saline Solution to Mitigate Proinflammatory and Degradative Responses of Articular Cartilage and Meniscus for Application to Arthroscopic Surgery

  • Lasun O. Oladeji
    Affiliations
    Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri, U.S.A.

    Thompson Laboratory for Regenerative Orthopaedics, University of Missouri, Columbia, Missouri, U.S.A.
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  • Aaron M. Stoker
    Affiliations
    Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri, U.S.A.

    Thompson Laboratory for Regenerative Orthopaedics, University of Missouri, Columbia, Missouri, U.S.A.
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  • James P. Stannard
    Affiliations
    Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri, U.S.A.

    Thompson Laboratory for Regenerative Orthopaedics, University of Missouri, Columbia, Missouri, U.S.A.
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  • James L. Cook
    Correspondence
    Address correspondence to James L. Cook, D.V.M., Ph.D., O.T.S.C., Orthopaedic Research Division, University of Missouri, Missouri Orthopaedic Institute (4028A), 1100 Virginia Ave., Columbia, MO 65212.
    Affiliations
    Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri, U.S.A.

    Thompson Laboratory for Regenerative Orthopaedics, University of Missouri, Columbia, Missouri, U.S.A.
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      Purpose

      This study was designed to evaluate differences in proinflammatory and degradative mediator production and extracellular matrix degradation from osteoarthritic knee articular cartilage and meniscus explants treated with either hyperosmolar saline or isotonic saline.

      Methods

      6 mm-diameter full-thickness explants were created from articular cartilage and menisci recovered after patients underwent knee surgery. One explant half was treated for 3 hours with hyperosmolar saline (600 mOsm/L) and the corresponding half with isotonic saline (300 mOsm/L). Explants were subsequently cultured for 3 days in tissue culture media. On day 3, media were collected for biomarker analyses. Results were normalized to tissue wet weight and analyzed statistically.

      Results

      Articular cartilage was collected from 10 patients (5 male, 5 female; mean age = 66.9 years) and menisci were collected from 8 patients (2 male, 6 female; mean age = 66 years). Articular cartilage media concentrations of monocyte chemoattractant protein-1 ( P = .001) and interleukin (IL)-6 ( P = .049) were significantly lower in explants treated with hyperosmolar saline. Meniscus media concentrations of prostaglandin E 2 ( P = .008), monocyte chemoattractant protein-1 ( P = .011), IL-6 ( P = .029), IL-8 ( P = .012), matrix metalloproteinase-2 ( P = .011), and glycosaminoglycan ( P = .008) were significantly lower in explants treated with hyperosmolar saline.

      Conclusions

      Treatment of cartilage and meniscus explants with hyperosmolar saline effectively mitigated key proinflammatory mediator production, as well as degradative mediator production and glycosaminoglycan loss from meniscus, with no detrimental effects noted compared to isotonic saline.

      Clinical Relevance

      These results suggest that hyperosmolar saline irrigation fluid may provide a safe alternative to standard isotonic saline irrigation fluid, and could mitigate untoward effects associated with inflammatory responses after standard-of-care knee arthroscopy.
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